| 2013/05/22 | Minnesota United |  | 0-1 |  | Des Moines Menace | | | | |
| | Rochester New York |  | 1-0 |  | Portland Phoenix | | | | |
| | Ocean City Nor´easters |  | 1-0 |  | Pittsburgh Riverhounds | | | | |
| | Austin Aztex |  | 0-2 |  | Wilmington Hammerheads | | | | |
| | San Antonio Scorpions |  | 2-2
(3-4
Pen.) |  | FC Tucson | | | | |
| | Portland Timbers S23 |  | 0-1 |  | Charleston Battery | | | | |
| | Los Angeles Blues |  | 5-1 |  | Ventura Fusion | | | | |
| | Fort Lauderdale Strikers |  | 1-1
(7-6
Pen.) |  | Laredo Heat | | | | |
| | Tampa Flames |  | 1-2 |  | Tampa Bay Rowdies | | | | |
| | Carolina RailHawks |  | 3-1 |  | Carolina Dynamo | | | | |
| | Richmond Kickers |  | 4-1 |  | Icon FC | | | | |
| | Ocala Stampede |  | 1-2 |  | Orlando City | | | | |
| | Charlotte Eagles |  | 3-0 |  | Seattle Sounders S23 | | | | |
| | Dayton Dutch Lions |  | 4-1
Pro. |  | Dearborn Stars | | | | |
| | Georgia Revolution |  | 2-3 |  | Atlanta Silverbacks | | | | |
| | Reading United |  | 1-0 |  | Harrisburg City Islanders | | | | |
16/05 - 23:00
17/05 - 15:00
17/05 - 15:45
17/05 - 19:00
